# Limit of Aluminum

Uses fluorescence spectroscopy after extraction with a chelating agent.

### Step-by-Step Instructions

1. Dissolve 20.0 g of Sodium Chloride in 100 mL of water and add pH 6.0 acetate buffer.
2. Extract the solution three times with a 0.5% solution of 8-hydroxyquinoline in chloroform.
3. Combine the extracts and measure the fluorescence (Excitation: 392 nm, Emission: 518 nm).

### Acceptance Criteria

- Fluorescence does not exceed that of the standard (NMT 0.2 ppm).

### Tips and Cautions

<div align="left" dir="ltr" id="bkmrk-category-guidance-ch"><table><colgroup><col width="312"></col><col width="312"></col></colgroup><thead><tr><th scope="col">Category

</th><th scope="col">Guidance

</th></tr></thead><tbody><tr><td>Chloroform Safety

</td><td>The Aluminum test uses chloroform, which is toxic and a suspected carcinogen. Use a fume hood and wear appropriate PPE.

</td></tr><tr><td>Contamination

</td><td>Use high-purity reagents and acid-washed glassware to avoid trace metal contamination.

</td></tr></tbody></table>

</div>