Limit of Aluminum

Uses fluorescence spectroscopy after extraction with a chelating agent. 
 Step-by-Step Instructions 
 
 
 Dissolve 20.0 g of Sodium Chloride in 100 mL of water and add pH 6.0 acetate buffer. 
 
 
 Extract the solution three times with a 0.5% solution of 8-hydroxyquinoline in chloroform. 
 
 
 Combine the extracts and measure the fluorescence (Excitation: 392 nm, Emission: 518 nm). 
 
 
 Acceptance Criteria 
 
 
 Fluorescence does not exceed that of the standard (NMT 0.2 ppm). 
 
 
 Tips and Cautions 
 
 
 
 
 
 Category 
 
 
 Guidance 
 
 
 
 
 
 
 Chloroform Safety 
 
 
 The Aluminum test uses chloroform, which is toxic and a suspected carcinogen. Use a fume hood and wear appropriate PPE. 
 
 
 
 
 Contamination 
 
 
 Use high-purity reagents and acid-washed glassware to avoid trace metal contamination.